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A protein repairing enzyme, PROTEIN L- ISOASPARTYL METHYLTRANSFERASE is involved in salinity stress tolerance by increasing efficiency of ROS-scavenging enzymes

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Title A protein repairing enzyme, PROTEIN L- ISOASPARTYL METHYLTRANSFERASE is involved in salinity stress tolerance by increasing efficiency of ROS-scavenging enzymes
 
Creator Ghosh, Shraboni
Kamble, Nitin Uttam
Majee, Manoj
 
Subject Salinity stress tolerance
PIMT
isoAsp
ROS
Antioxidant enzymes
Arabidopsis
 
Description Accepted date: 2 September 2020
Saline conditions can significantly affect plant growth and development, leading to massive reduction in crop
yield. Herein, we show that a protein repairing enzyme PROTEIN L-ISOASPARTYL METHYLTRANSFERASE
imparts salinity stress tolerance in Arabidopsis thaliana by repairing deleterious isoAsp accumulation during
salinity stress. We demonstrate that salinity stress accelerates isoAsp accumulation in proteins and also induces
PIMT activity in Arabidopsis. Transcript analysis indicates that both PIMT1 and PIMT2 are upregulated in
response to salinity stress. Subsequent functional analysis reveals that PIMT1 and PIMT2 overexpression lines are
tolerant, while RNAi lines are hyper sensitive to salinity stress in comparison to wild type (WT). Biochemical
analyses of thesePIMT transgenic lines also reveals that compromised salinity tolerance of RNAi lines are linked
to increased isoAsp accumulation, while improved tolerance of overexpression lines is associated with reduced
isoAsp accumulation in proteins. Histochemical and biochemical studies further confirm lower accumulation of
ROS and reduced lipid peroxidation in PIMT overexpression lines, while increased ROS accumulation and
increased lipid peroxidation in RNAi lines as compared to WT under salinity stress. Interestingly, PIMToverexpression lines exhibit improved antioxidant enzyme efficiency, while RNAi lines display compromised antioxidant enzyme efficacy as compared to WT type plants. Our study suggests that PIMT improves salinity stress
tolerance by restricting salt induced-excess ROS accumulation possibly by repairing isoAsp mediated protein
damage of antioxidant enzymes. Our study can be utilized for enhancing salinity stress tolerance of economically
important crops.
This work was supported by a grant from Department of Biotechnology (BT/PR8000/BRB/10/1210/2013), Government of India and core grant of National Institute of Plant Genome Research, New Delhi, India. SG and NUK thank University Grant Commission, Government of India and NIPGR, for research fellowship. We thank technicians of NIPGR central instrumentation facility. We thank Metabolomics facility at NIPGR for ICP-MS analysis (BT/INF/22/SP28268/2018). The authors are thankful to DBT-eLibrary Consortium (DeLCON) for providing access to e-resources.
 
Date 2020-09-21T09:58:56Z
2020-09-21T09:58:56Z
2020
 
Type Article
 
Identifier Environmental and Experimental Botany, 180: 104266
0098-8472
https://doi.org/10.1016/j.envexpbot.2020.104266
https://www.sciencedirect.com/science/article/pii/S0098847220302926
http://223.31.159.10:8080/jspui/handle/123456789/1097
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.