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Magnaporthe oryzae MoNdt80 is a transcriptional regulator of GlcNAc catabolic pathway involved in pathogenesis

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Title Magnaporthe oryzae MoNdt80 is a transcriptional regulator of GlcNAc catabolic pathway involved in pathogenesis
 
Creator Bhatt, Dharmendra Nath
Ansari, Sekhu
Kumar, Anil
Ghosh, Sumit
Narula, Alka
Datta, Asis
 
Subject N-Acetylglucosamine
Rice blast disease
Magnaporthe oryzae
Ndt80
Transcriptional regulation
Host-pathogen interaction
 
Description Accepted dated: 3 July 2020
Availability and efficient utilization of host-derived nutrients by pathogens decide the fate of host-pathogen interaction. In Magnaporthe oryzae, N-acetylglucosamine (GlcNAc) catabolic pathway was found essential for successful host colonization and pathogenicity. GlcNAc catabolic enzymes hexokinase, GlcNAc-6-phosphate deacetylase (MoDac) and GlcN-6-phosphate deaminase (MoDeam) are encoded in a genomic cluster in M. oryzae and several phytopathogenic fungi. However, transcriptional regulation of GlcNAc catabolic pathway was not understood. We identified a conserved Ndt80/PhoG-like transcriptional regulator as a part of the GlcNAc catabolic gene cluster in M. oryzae and other fungi. We found that MoNdt80 is essential for GlcNAc utilization and pathogenicity of M. oryzae. Unlike WT, ΔMoNdt80 failed to induce transcription of GlcNAc catabolic pathway genes in response to GlcNAc. MoNdt80 could bind to a specific cis-acting consensus sequence GNCRCAAA[AT], present in the promoter of MoDac, MoDeam and β-hexosaminidase (MoHex). Further, comparative RNA-sequencing analysis using WT and ΔMoNdt80 revealed a large set of GlcNAc responsive genes that are under the transcriptional control of MoNdt80. These genes encoded GlcNAc catabolic enzymes, transporters and cell wall degrading enzymes which are required for hyphal growth expansion during host colonization. Overall, these results suggest MoNdt80 mediated transcriptional regulation of GlcNAc catabolic pathway is essential for successful host colonization and pathogenesis.
We thank Dr. T. R. Sharma and Dr. K. V. Prabhu (Indian Agricultural
Research Institute, New Delhi) for providing the wild-type strain of M.
oryzae and rice cultivar Pusa Basmati-1 respectively. We are also
thankful to the Fungal Genetics Stock Center, USA, for pCB1003 and
pCB1532 plasmids. This work is financially supported by Department of
Biotechnology and the Core Grant of National Institute of Plant Genome
Research, New Delhi, India. DNB acknowledges University Grants
Commission for the Senior research fellowship. SA acknowledges Indian
council of medical research for the Senior research fellowship.
 
Date 2020-07-29T10:08:50Z
2020-07-29T10:08:50Z
2020
 
Type Article
 
Identifier Microbiological Research, 239: 126550
0944-5013
https://doi.org/10.1016/j.micres.2020.126550
https://www.sciencedirect.com/science/article/pii/S0944501320304183
http://223.31.159.10:8080/jspui/handle/123456789/1083
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.