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Restriction enzyme analysis of satellite DNA components from poultry genome

Indian Agricultural Research Journals

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Title Restriction enzyme analysis of satellite DNA components from poultry genome
 
Creator Sharma, Deepak
Rao, K B C Appa
Rawat, A K
 
Subject Poultry genome; Repetitive DNA; Restriction enzyme
 
Description The four economically important species of poultry, viz, chicken (Gallus domesticus), quail (Coturnux japonica), gunea fowl (Numida meleagris) and turkey (Meleagris gallopavo) were studied for both mdividual and comparatIve restriction profile analysis of repetitive DNA using 15 restriction enzymes. Genomic DNA isolated from 10 birds (5 of either sex) from each species were digested satisfactorily with enzymes which include both tetra and hexa cutters. No discrete bands could be observed with any of the enzymes. However, some enzymes (Msp I, Taq I, Apa I, Dra I, Eeo RI, Hind III, Nde I, Pst I and Sac I) yielded few Feeble bands within the smear, No distinct species or sex specific differences were observed with respect to repetitive DNA profile in these poultry species, The results, therefore clearly demonstrated that repetitive DNA component seems to exist moderately and thereby constitute a small proportion to total genome in different poultry species studied.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-07-05
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/21222
 
Source The Indian Journal of Animal Sciences; Vol 69, No 9 (1999)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/21222/10710
 
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