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Antibiotic‑active heterotrophic Firmicutes sheltered in seaweeds: can they add new dimensions to future antimicrobial agents?

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Relation http://eprints.cmfri.org.in/15808/
https://link.springer.com/article/10.1007/s00203-022-02784-2
https://doi.org/10.1007/s00203-022-02784-2
 
Title Antibiotic‑active heterotrophic Firmicutes sheltered in seaweeds: can they add new dimensions to future antimicrobial agents?
 
Creator Chakraborty, Kajal
Varghese, Chesvin
Asharaf, Sumayya
Chakraborty, Rekha D
 
Subject Seaweed
Microbiology
Fish Biotechnology
Algae
 
Description Appearance of drug-resistant microorganisms prompted researchers to unravel new environments for development of novel antimicrobial agents. Culture-supported analysis of heterotrophic bacteria associated with seaweeds yielded 152 strains, in that larger share of the isolates was embodied by Bacillus atrophaeus SHB2097 (54%), B. velezensis SHB2098 (24%), B. subtilis SHB2099 (12%), and B. amyloliquefaciens SHB20910 (10%). One of the most active strains characterized as B. atrophaeus SHB2097 (MW821482) with an inhibition zone more than 30 mm on spot-over-lawn experiment, was isolated
from a seaweed Sargassum wightii, was selected for bioprospecting studies. Signifcant antibacterial potential was displayed by bacterial organic extract against vancomycin-resistant Enterococcus faecalis, Pseudomonas aeruginosa, methicillinresistant Staphylococcus aureus, and Klebsiella pneumonia with minimum inhibitory concentration 6.25 µg/mL and comparable to the antibiotics ampicillin and chloramphenicol. The genes of type 1 pks (MZ222383, 700 bp) and hybrid nrps/ pks (MZ222389, 1000–1400 bp) of B. atrophaeus MW821482 could be amplifed. The bacterium displayed susceptibility/ to the commercially available antibiotic agents, and was negative for the pore-forming non-hemolytic hemolysin BL (hbl) and enterotoxin (nhe) genes, and therefore, was not pathogenic. The bacterium was found to possess genes (1000–1400 bp) involved in the biosynthesis of siderophore-class of compounds (MZ222387 and MZ222388) that showed 99% of similarity in BLAST search, and showed production of siderophore. Noteworthy antibacterial activities against clinically important pathogenic bacteria in conjunction with occurrence of genes coding for antimicrobial metabolites inferred that the marine
heterotrophic bacterium B. atrophaeus SHB2097 could be used for the development of antibacterial agents against the
emerging antibiotic resistance.
 
Publisher Springer
 
Date 2022
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/15808/1/Archives%20of%20Microbiology_2022_Kajal%20Chakraborty.pdf
Chakraborty, Kajal and Varghese, Chesvin and Asharaf, Sumayya and Chakraborty, Rekha D (2022) Antibiotic‑active heterotrophic Firmicutes sheltered in seaweeds: can they add new dimensions to future antimicrobial agents? Archives of Microbiology. pp. 1-14.