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A comparative study of outer membrane proteins of selected Brucella strains

Indian Agricultural Research Journals

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Title A comparative study of outer membrane proteins of selected Brucella strains
 
Creator ISLOOR, S
SURYANARAYANA, V V S
RAO, M SATYANARAYANA
RAJASEKHAR, M
 
Subject Brucella, Outer membrane proteins, SDS-PAGE
 
Description Outer membrane proteins (OMPs) of Brucella abortus strains (99,544 and 19), Brucella melitensis 16M and Brucella suis 1330 were isolated by differential centrifugation method, Based on sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE) profiles, Brucella OMPs were classified into 3 distinct molecular weight (MW) ranges, viz, low MW (14-30 kDa), moderate MW (39-60 kDa) and high MW (68-93 kDa), Polypeptides above 60 kDa were not observed in B, abortus 544. The moderate MW bands were more varied in their migration and relative quantity, Silver staining confirmed the presence of polysaccharide moieties in major bands at position 39,2-47,3 kDa,The subtle differences observed in SDS-PAGE analysis of Brucella OMPs did not represent rei iable marker for identification of Brucella species.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor
 
Date 2014-01-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/36470
 
Source The Indian Journal of Animal Sciences; Vol 71, No 3 (2001)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/36470/16242
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences