Marine Macroalgal Polygalactan-Built Nanoparticle Construct for Osteogenesis
CMFRI Repository
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Relation |
http://eprints.cmfri.org.in/15148/
https://doi.org/10.1021/acs.biomac.1c00270 https://doi.org/10.1021/acs.biomac.1c00270 |
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Title |
Marine Macroalgal Polygalactan-Built Nanoparticle Construct for Osteogenesis |
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Creator |
Chakraborty, Kajal
Antony, Tima Dhara, Subhajit |
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Subject |
Bioactive compound
Fish Biotechnology Algae |
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Description |
Naturally derived polysaccharide biopolymer-based nanoparticles with their size and drug release potentials have appeared as promising biomaterials for osteogenic differentiation. A metallic nanoparticle (GS-AgNP) prepared from a sulfated polygalactan characterized as →3)-2-O-methyl-O-6-sulfonato-β-d-galactopyranosyl-(1 → 4)-2-O-methyl-3,6-anhydro-α-d-galactopyranose-(1→ isolated from the marine macroalga Gracilaria salicornia exhibited a prospective osteogenic effect. Upon treatment with the studied GS-AgNP, alkaline phosphatase activity (88.9 mU/mg) was significantly elevated in human mesenchymal osteoblast stem cells (hMSCs) compared to that in the normal control (33.7 mU/mg). A mineralization study of GS-AgNPs demonstrated an intense mineralized nodule formation on the hMSC surface. A fluorescence-activated cell sorting study of osteocalcin and bone morphogenic protein-2 (BMP-2) expression resulted in an increased population of osteocalcin (78.64%) and BMP-2-positive cells (46.10%) after treatment with GS-AgNPs (250 μg/mL) on M2 macrophages. A time-dependent cell viability study of GS-AgNPs exhibited its non-cytotoxic nature. The studied polygalactan-built nanoparticle could be developed as a promising bioactive pharmacophore against metabolic bone disorder and the treatment for osteogenesis therapy.
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Date |
2021
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Type |
Article
PeerReviewed |
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Format |
text
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Language |
en
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Identifier |
http://eprints.cmfri.org.in/15148/1/Biomacromolecules_2021_Kajal%20Chakraborty.pdf
Chakraborty, Kajal and Antony, Tima and Dhara, Subhajit (2021) Marine Macroalgal Polygalactan-Built Nanoparticle Construct for Osteogenesis. Biomacromolecules, 22. pp. 2197-2210. |
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