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Quantification of rumen undegradable protein fractions of conventional and non conventional protein supplements by SDS-PAGE

Indian Agricultural Research Journals

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Title Quantification of rumen undegradable protein fractions of conventional and non conventional protein supplements by SDS-PAGE
 
Creator Wadhwa, M
Kaur, N
Bakshi, M P S
 
Subject Conventional; In-sacco rumen degradability; Non-conventional; Protein fractions; Protein supplements; SDS-PAGE
 
Description Conventional [solvent extracted GNC and mechanically extracted mustard cake (MC)], and non-conventional protein supplements [machanically extracted castor oilseed cake (COSC), un-decorticated solvent extracted neem seed cake (NSC) and corn gluten meal (CGM)] before and after rumen incubations for different intervals were subjected to sequential fractionation into globulin, albumin, prolamin and glutelins on the basis of solubility. The polypeptides in globulin, prolamin and glutelin fraction of groundnut cake with molecular weight 35, 15, 16 and 19 KDA, respectively, were highly resistant to microbial degradation even after 24h or rumen incubation. The degradation pattern of mustard cake revealed that subunits of globulin, prolamin and glutelins with MW of 14–33, 14 and 31 KDA, respectively, were highly resistant to microbial degradation. In NSC subunits with MW 124 (in globulin), 30 (in albumin), 66 (in prolamin) and 54 kDa (in glutelin) were resistant to microbial degradation. The subunits in prolamin fraction of CGM with MW 18, 24 and 43 kDa and that in glutelin fraction with MW of 22 and 60 kDa were found to be highly resistant to microbial degradation. The results conclusively revealed that in majority of the cases, the low molecular weight polypeptides in the tested protein supplements showed resistance to rumen microbial fermentation, even after 48h of rumen incubation.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-09-10
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/23660
 
Source The Indian Journal of Animal Sciences; Vol 82, No 9 (2012)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/23660/11344
 
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