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Exogenous ß-galactosidase decreased lactose in caprine mammary epithelial cells

Indian Agricultural Research Journals

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Title Exogenous ß-galactosidase decreased lactose in caprine mammary epithelial cells
 
Creator TIAN, LEI
LI, YE
ZHANG, LI
LI, QING-ZHANG
 
Subject ß-galactosidase, Lactose, Lactose intolerance, Mammary epithelial cells
 
Description This study was conducted to express exogenous ß-galactosidase and make lactose hydrolyzed in mammary epithelialcells which could provide a valuable way for obviate lactose intolerance.PCR was used to get the 5' flanking regulatory region of bovine alpha S1 casein gene (1.2kb) from the genomic DNA andconstruct the vector. ß-galactosidase was expressed under the control of 5' flanking regulatory regions of bovine alpha S1casein gene (1.2kb) in caprine mammary epithelial cells (CMECs). LacZ gene expression specificity was detected by5bromo-4-chloro–3-indolyl-ß-D-galactoside (X-gal) staining of the ß-galactosidase activity. In the cell culture supernatantand cell extracts, ß-galactosidase was detected within 24 to 144 h after transfection. ß-galactosidase of the cell extractshad a gradually decrease tendency in 24 to 72 h. In contrast, ß-galactosidase of cell culture supernatant had a gradualincrease tendency. In the cell extracts, ß-galactosidase could be detected in 1 to 3 generation. HPLC showed that thelactose concentration of CMECs was reduced by ß-galactosidase and the effects held significantly in 24 to 144 h posttransfection.ß-galactosidase could express in CMECs and significantly reduced the lactose concentration of CMECs,these findings would provide an available way for obviate lactose intolerance.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-02-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/27252
 
Source The Indian Journal of Animal Sciences; Vol 83, No 2 (2013)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/27252/12398
 
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