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Enzyme-linked immunosorbent assay for detection of Mycobacterium bovis antibodies in cattle

Indian Agricultural Research Journals

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Title Enzyme-linked immunosorbent assay for detection of Mycobacterium bovis antibodies in cattle
 
Creator GUPTA, V K
RAM, G C
 
Subject Mycobacterium bovis, Enzyme -linked immunoserbent arrays
 
Description The major and general problem in serodiagnosis of bovine tuberculosis is the grade of specificity of the test. Lack of specificity of bovine PPD caused unacceptable number of the false positive results. In this study the usefulness of enzyme linked immunosorbent assay (ELISA) for the diagnosis of bovine tuberculosis is further evaluated. A total of 102 cows (50 from lzatnagar farm and 52 from Pantnagar farm). were tested for the presence of anti Mycobacterium bovis antibodies in their sera. Bovine PPD and M. bovis specific glycolipid neoantigen (MB-O-BSA) were used as antigens in ELISA. The percentages of seropositive animals were 94% and 100% with bovine PPD in Izatnager and Pantnagar, whereas only 50% and 82.6% animals reacted to neoantigen in ELISA in both the farms respectively. It was observed that use of speciesspecitic antigens in ELISA may reduce the number of false reactor and revolutionize the serodiagnosis of tuberculosis in cattles.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-10-18
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/34048
 
Source The Indian Journal of Animal Sciences; Vol 67, No 1 (1997)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/34048/15083
 
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