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Polymorphism at growth hormone gene in Malabari goats investigated by PCR-RFLP

Indian Agricultural Research Journals

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Title Polymorphism at growth hormone gene in Malabari goats investigated by PCR-RFLP
 
Creator CHITRA, R
ARAV1NDAKSHAN, T V
 
Subject Growth hormone gene; Linkage; Malabari goats; Mspl polymorphism; Milk; SNF percentage
 
Description The genetic polym~rphismw as investigated at the third intron of growth hormone (GH) gene in Malabari goats using the PCR-RFLP method. A random sample of 196 genetically unrelated Malabari does in second lactation belonging to 3 different populations were analysed. The successful amplification of the expected fragment indicated the suitability of bovine primers for goats and conservation of DAN sequence in related species. The digestian of the amplified product with MspI restriction enzyme revealed 2 alleles, viz. MspI (+) Mspl (-) with frequencies of 0.70 and 0.30 respectively. There was no significant variation in GHIMspl gene frequencies among the different populations, indicating the homogeneity of Malabari goats as a breed though they differ significantly with respect tomorphological and other phenotypic characters. The GHIMspI (+I-) genotype was associated with a significantly high (P
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor ICAR
 
Date 2011-01-21
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/3753
 
Source The Indian Journal of Animal Sciences; Vol 74, No 12 (2004)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/3753/1522
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences