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PCR-RFLP and nucleotide sequencing of mx1 gene in chicken

Indian Agricultural Research Journals

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Title PCR-RFLP and nucleotide sequencing of mx1 gene in chicken
 
Creator MISHRA, CHINMOY
DAS, DHARMESWAR
KUMAR, PUSHPENDRA
SHARMA, ARJAVA
 
Subject Aseel; Chicken; Mx1 gene; Polymorphism; PCR-RFLP; Sequencing
 
Description Mx1 gene is an interferon induced gene which inhibits the proliferation of single stranded RNA viruses including influenza virus. Single nucleotide polymorphism in the Mx1 gene was reported to be associated with resistance to influenza virus. However, the indigenous breeds of India are not screened for Mx1 gene previously. In the present investigation partial intron 13 and partial exon 14 of Mx1 gene was studied using PCR-RFLP technique in 153 Aseel breed of chicken. A 100 bp PCR amplified products were digested with Rsa I restriction enzyme which revealed 2 genotypes (RS and SS) and 2 different alleles (R and S). The RR genotype could not be found in this population. The genotype frequency was 0.655 and 0.345 for RS and SS genotypes and 0.33 and 0.67 for R and S alleles, respectively. PCR product of both the alleles were sequenced and submitted to NCBI GenBank data. The analysis of sequence data by DNASTAR programme revealed differences at one (72nd) position in 100 bp fragment. There is an urgent need of wide search for detection of polymorphism in Mx1 gene of chicken and its possible association with the traits of economic importance.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-07-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/7732
 
Source The Indian Journal of Animal Sciences; Vol 81, No 7 (2011)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/7732/3124
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences