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Duplex PCR for specific detection of Escherichia coli and its differentiation from other Enterobacteriaceae

Indian Agricultural Research Journals

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Title Duplex PCR for specific detection of Escherichia coli and its differentiation from other Enterobacteriaceae
 
Creator MITRA, SUSWETA DAS
DAS, WILFRED ANTHONY
TEWARI, RITUPARNA
C, NIMITA VENUGOPAL
MANI, BHUVANA
NATESAN, KRITHIGA
SHOME, BIBEK RANJAN
RAHMAN, HABIBUR
 
Subject Duplex polymerase chain reaction, Enterobacteriaceae, Escherichia coli
 
Description Escherichia coli is a normal inhabitant of gastrointestinal tract of humans and animals and one of the most important causes of bovine mastitis. Definitive identification of E. coli from other members of Enterobacteriaceae remains ambiguous. The present study describes a duplex PCR, targeting 2 housekeeping genes, the lacy (lactose permease) and phoA (alkaline phosphatase) for the reliable detection of E. coli that enables its differentiation from biochemically and phylogenetically related bacteria. The assay was evaluated employing 19 ATCC (American type culture collection) reference strains of Enterobacteriaceae family. Validation of the assay with E. coli (154) isolated from milk and faeces rendered the assay to be specific. The results suggest that the technique can be used for accurate detection of E. coli and thus can be adapted for testing bacteriological safety of milk, for field applications, and in laboratories handling clinical samples. This PCR (polymerase chain reaction) can successfully distinguish E. coli including E. coli O157 from Shigella spp and other related enterobacteria, emphasizing its relevance and utility in studies related to E. coli infection.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2015-08-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/50931
 
Source The Indian Journal of Animal Sciences; Vol 85, No 8 (2015)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/50931/21650
 
Rights Copyright (c) 2015 The Indian Journal of Animal Sciences