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Development of 12 polymorphic EST-SSR for endangered fish, Hucho taimen (Pallas)

Indian Agricultural Research Journals

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Title Development of 12 polymorphic EST-SSR for endangered fish, Hucho taimen (Pallas)
 
Creator JUN, WANG
YOU-YI, KUANG
 
Subject cDNA library; EST-SSR; Genetic diversity; Hucho taimen (Pallas)
 
Description Hucho taimen (Pallas) is an indigenous and endangered cold-water fish in China. To access the genetic diversity of wild breeding parents of this species and promote its breeding selection program, 12 polymorphic EST-SSR markers were developed from its cDNA library by (CA)16 probe and were analyzed on 66 wild breeding parents. The number of alleles per locus (Na), expected heterozygosity (He) and polymorphism information content (PIC) ranged from 2 to 9, 0.2019 to 0.8542 and 0.1802 to 0.8312 respectively. In total, a new and economical method for EST-SSR was established. The EST-SSR markers characterized in this study will strengthen our interpretation of Hucho taimen and facilitate its breeding selection program to improve the endangered statue of this species.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-03-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4831
 
Source The Indian Journal of Animal Sciences; Vol 81, No 3 (2011)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4831/2009
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences