Record Details

Optimal conditions for assay of haemolytic component of camel complement in classical pathway

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Optimal conditions for assay of haemolytic component of camel complement in classical pathway
 
Creator CHOPRA, V
KASHYAP, S K
 
Subject Camel, Camel complement, Classical pathway, Haemolytic component
 
Description Haemolytic complement of camel serum was assayed under standardized conditions. CH50 titres in the range of 110.03-417.8 units ml-1 of camel serum with a mean of 197.8 were obtained. Effect of various variables on the lytic activity of camel complement in classical pathway was studied. The standard system of titrating the HC level consisted of rabbit erythrocytes sensitized with goat haemolysin as indicator system, veronal buffer saline, pH 7.3, ionic strength 0.15M, Ca2+ and Mg2+ concentrations of 4.10-4 and 1.10-3M, respectively. Incubation at 37°C for 60 min gave the highest HC activity. Higher Na2 EDT A concentrations than 5.10-4 M inhibited haemolytic activity to undetectable levels.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2014-01-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/37198
 
Source The Indian Journal of Animal Sciences; Vol 71, No 11 (2001)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/37198/16619
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences