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Microsatellite analysis after long term selection for egg production in Rhode Island Red chicken

Indian Agricultural Research Journals

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Title Microsatellite analysis after long term selection for egg production in Rhode Island Red chicken
 
Creator DAS, ANANTA KUMAR
KUMAR, SANJEEV
RAHIM, ABDUL
 
Subject Allele frequency, Effective number of alleles, Line-specific alleles, Microsatellites, RIR chicken lines, Shannon’s information index
 
Description This investigation aimed to analyze microsatellites after long term selection for egg production in the selected line (RIRS) of Rhode Island Red chicken and its control line (RIRC) maintained at the institute. Genomic DNA samples isolated from 24 randomly selected birds of RIRS and RIRC line were investigated at 24 microsatellite loci. Microsatellite alleles were separated on 6% urea-PAGE and their sizes were estimated with the help of Gel Doc 2000 system. Allelic data was analyzed. Analysis revealed 2 to 7 alleles in RIRS and 2 to 9 alleles in RIRC line across 24 loci with their sizes ranged from 84 to 276 bp. Observed number of alleles per locus was 4.04±0.23 in RIRS and 4.42±0.33 in RIRC. Allele frequency ranged from 0.083 to 0.667 in RIRS and 0.042 to 0.833 in RIRC. Approximately 34.02% of alleles in RIRS and 39.62% alleles in RIRC were line specific. The frequencies of the specific alleles ranged from 0.083 to 0.667 in RIRS and 0.083 to 0.883 in RIRC. Line specific alleles with higher frequencies can be used in line identification. Corresponding effective number of alleles and Shannon’s information index averaged 3.32±0.19 and 1.25±0.06 in RIRS and 3.66±0.32 and 1.30±0.08 in RIRC. These diversity estimates indicated that the control line was more diverse than the selected line and certain specific microsatellite alleles were getting fixed in the selected line.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2015-11-06
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/53280
 
Source The Indian Journal of Animal Sciences; Vol 85, No 11 (2015)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/53280/22502
 
Rights Copyright (c) 2015 The Indian Journal of Animal Sciences