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Genomic restriction fragment analysis of fowlpox-virus vaccine strain and field isolates using EcoRI, Sal I and Pst I restriction endonucleases

Indian Agricultural Research Journals

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Title Genomic restriction fragment analysis of fowlpox-virus vaccine strain and field isolates using EcoRI, Sal I and Pst I restriction endonucleases
 
Creator MISHRA, S S
MALLICK, B B
 
Subject —
 
Description Fow-pox-virus [VR] vaccine strain and 2 field isolates were propagated in chicken-embryofibroblast-cell culture and purified by sucrose gradient ultracentrifugation. Transmission eleclronmicroscopy revealed the purified virus showing characteristic fowlpox-virus morphology with bilayer structure and surface tubular clements. The viral DNA was extracted and analysed after digestion with EcoRl, Sal I and Pst I restriction cndonucleases. BeoRI digestion produced 24 well resolved fragments with some comigrating_ fragments and analysis on a seperate gel revealed 40 restriction fragments. Sal I and Pst I digestion revealed a large unresolved fragments with 4 and 18 well resolved fragments respectively. The 3 viruses showed similar electrophoretic pattern of DNA restriction fragments indicating them to be genetically almost similar.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-10-14
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/33960
 
Source The Indian Journal of Animal Sciences; Vol 65, No 12 (1995)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/33960/15057
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences