Record Details

Development of water buffalo (Bubalus bubalis) embryos after parthenogenetic activation of oocytes with calcium ionophore, ethanol and 6-dimethylaminopurine

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Development of water buffalo (Bubalus bubalis) embryos after parthenogenetic activation of oocytes with calcium ionophore, ethanol and 6-dimethylaminopurine
 
Creator VERMA, V
GAUTAM, S K
SINGH, B
KUMAR, M
CHAUHAN, M S
 
Subject Blastocyst; Ionophore; Parthenote
 
Description The present study was carried out to investigate the effects of various activation treatments on the parthenogenetic development of buffalo oocytes and their comparison with the IVF embryos and to investigate the mean total cell number per blastocysts produced. The rates of cleavage and blastocyst development were significantly higher when the oocytes were activated by combined treatment of calcium ionophore (A23187) and ethanol, followed by 6- dimethylaminopurine (6-DMAP) than those activated by individual treatments with these chemicals, as well as blastocysts produced through IVF. Our results showed significant difference in the number of blastomeres between parthenotes and IVF buffalo embryos. Our results can be used to establish effective protocols for activation of buffalo oocytes and development of embryos which can be used to study mechanisms involved in the initiation of the embryonic development, and derivation of embryonic stem (ES) cells for various applications.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-07-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/7717
 
Source The Indian Journal of Animal Sciences; Vol 81, No 7 (2011)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/7717/3114
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences