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Single nucleotide polymorphisms a-lactalbumin gene in cattle and their effect in mature protein

Indian Agricultural Research Journals

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Title Single nucleotide polymorphisms a-lactalbumin gene in cattle and their effect in mature protein
 
Creator Ramesha, K P
Gowda, Shivraj
R C, Nagasuma
Rao, Mrs
 
Subject Alpha lactalbumin gene, Cattle, Protein, Single nucleotide polymorphism (SNP)
 
Description Indigenous cattle breeds of Southern India (Amrithmahal, Krishna Valley, Haliikar, Deoni, Ongole and Malnad Gidda) and Holstein crosses (HF x Sahiwal) were screened for single nucleotide polymorphisms (SNPs) in the coding sequence of the bovine alpha lactalbumin gene. SNPs at 4 positions (772, 775, 792 and 851) in exon 1 and 3 positions at 1231, 1264 and 1335 in exon 2 were detected by SSCP analysis and the same were confirmed by direct sequencing of the PCR products. The amino acid change in the protein due to SNPs in the coding region were predicted based on Transeq predict database ofEMBL-EBI. The polymorphism at positions 792, 1231 and 1335 were silent polymorphisms in the coding region of the gene, while the SNPs observed at positions 772, 775, 851 and 1264 are expected to bring about amino acid changes (S~T, F~L, R~Q and I~V respectively) in the protein. The effect of the SNPs in the mature protein was interpreted based on the crystal structure of bovine a-LA. 
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-03-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4420
 
Source The Indian Journal of Animal Sciences; Vol 76, No 10 (2006)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4420/1739
 
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