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Polymorphism and sequence analysis of growth hormone gene and its relationship with body weight in meat type of chicken

Indian Agricultural Research Journals

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Title Polymorphism and sequence analysis of growth hormone gene and its relationship with body weight in meat type of chicken
 
Creator SADEGH, M EKHTIARI
NAGARAJA, C S
JAYASHANKAR, M R
MURTHY, H N
 
Subject Body weight; cGH gene; Polymorphism; PCR-RFLP; Sequencing
 
Description The growth hormone gene is a candidate gene controlling the metabolic and physiologic actions. Blood and feather samples were collected from 200 individuals of birds and genomic DNA was extracted using salting out technique. DNA fragment with 776 bp at growth hormone gene was amplified using polymerase chin reaction (PCR).The products from each strain (New Genotype, Punjabi Broiler-2, Indian Curnish-3 and University Male Line) were digested with MspI. In total, 4 RFLP pattern were observed. The genotypic frequencies obtained for different strains were tested using chi-square test. The differences among genotypes for all the 4 strains were significant. The overall allele frequency for A, B and C alleles were 0.495, 0.110 and 0.395 respectively. Correlation of cGH/MspI patterns with sixth week body weight was estimated. There were significant differences among 4 strains for body weight. Nucleotide sequencing of the amplified fragment of GH gene of 4 strains were done and submitted to the NCBI Genbank (accession nos JN403372 and JN403373). The nucleotide sequence analysis indicated that the per cent similarity of GH gene fragment of 4 strains were more than 99. In this study result also showed that there is a scope for using growth hormone gene as a candidate gene for growth traits.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-12-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/25680
 
Source The Indian Journal of Animal Sciences; Vol 82, No 12 (2012)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/25680/11877
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences