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Adaptation of vaccine strain of duck plague virus in chicken embryo fibroblast cell culture

Indian Agricultural Research Journals

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Title Adaptation of vaccine strain of duck plague virus in chicken embryo fibroblast cell culture
 
Creator DOLEY, MONUJ
DAS, S K
BARMAN, N N
RAJBONGSHI, G
 
Subject AGPT, Cell culture adaptation, Chicken fibroblast cell culture, Duck plague virus, PCR
 
Description The duck plague vaccine virus was adapted to grow in chicken fibroblast cell culture. Adaptation of the virus in chicken fibroblast cell culture was confirmed by detection of viral infection in infected cells by PCR and AGPT. In PCR, amplification of DNA dependent DNA polymerase gene of duck plague virus yielded 446 bp in vaccine virus against no amplification in negative template control. Total time required to complete the PCR including extraction of viral DNA is about 5 h. On the other hand in AGPT, faint precipitation lines were observed following 24 h and distinct bands were seen after 48 h of incubation at room temperature. The precipitate lines were obtained with acetate (pH 5.6) and phosphate (pH 7.2) buffer incorporating additional 8% NaCl. The cell adapted duck plague virus in the present study provides a scope to undertake further research on duck plague in India towards development of cell culture vaccine for use in the country.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-09-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/33009
 
Source The Indian Journal of Animal Sciences; Vol 83, No 9 (2013)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/33009/14624
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences