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Fractionation and immunoreactivity of Mycobacterium hovis AN5 culture filtrate antigens

Indian Agricultural Research Journals

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Title Fractionation and immunoreactivity of Mycobacterium hovis AN5 culture filtrate antigens
 
Creator GUPTA, V C
RAM, G C
 
Subject Culture filtrate antigcns, ELISA, Mycobacterium bOLlis AN5
 
Description Protein secreted by Mycobacterium species have been suggested as major immune targets, Four weeks old M, bovis AN5 culture filtrate protein antigens were fractionated by gel permeation and anion exchange chromatography, These fractionoted antigcns WCIC evaluated for theirreactivity to anti M bovis AN5 antiserum in ELISA, Out of several fractionated antigens, the more reactive antigens were heterogenous polypeptides of 46,42,40 and 38 kDa and homogenous polypeptides of 48 and 17.4 kDa molecular masses, Western immunoblot analysis of crudc culturc filtrate antigens revealed 9 reactive bands of 74,48,46,43, 38,23,21.4,17,4 and 14 kDa molecular masses. Most of the fretionated culture fillmtc antigens showcd cross- reactivity with other Mycobacterium species in ELISA except 17.4 kDa antigen that showed species specificity to M. bovis AN5, It is concluded that some of the culture filtrate antigens are highly immunogenic for humoral response.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2014-01-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/36231
 
Source The Indian Journal of Animal Sciences; Vol 70, No 10 (2000)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/36231/16080
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences