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Cryopreservation of fish gametes and embryos

Indian Agricultural Research Journals

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Title Cryopreservation of fish gametes and embryos
 
Creator DIWAN, A D
AYYAPPAN, S
LAL, K K
LAKRA, W S
 
Subject Cryopreservation; Egg; Embryos; Fish; Sperm
 
Description Cryopreservation of gametes is one of the important ex situ methods of conservation of germplasm and has wide ranging applications in aquaculture and fisheries management. Though sperm cryopreservation has been a success in fishes, yet developing successful protocols for eggs and embryo cryopreservation still remains elusive. Despite successful sperm cryopreservation protocol known for more than 200 fish species, the adoption of the technique at commercial level for fish seed production has been limited. High degree of variability in the procedural requirements and success not only between the species but at times within the species, is considered one of the limiting factors in its utilization. The present paper records the status of gamete cryopreservation in fish species. The paper provides a comprehensive review of various aspects of milt cryopreservation such as milt collection, sperm quality and viability assessment, extender compositions, cryoprotectants equilibration periods, freezing rates, thawing of cryopreserved milt and fertilization of eggs and ultrastructural studies on damages in cryopreserved spermatozoa. The paper also provides a brief review of cryopreservation of fish embryos and embryonic stem cells.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor ICAR
 
Date 2010-10-05
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/1340
 
Source The Indian Journal of Animal Sciences; Vol 80, No 4 (2010): (Suppl. 1)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/1340/436
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences