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Amplification and cloning of exon regions from 1-4 of cathepsin B-like cysteine protease gene of Haemonchus contortus and immune response in lambs against cathepsin B-like cysteine protease fraction

Indian Agricultural Research Journals

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Title Amplification and cloning of exon regions from 1-4 of cathepsin B-like cysteine protease gene of Haemonchus contortus and immune response in lambs against cathepsin B-like cysteine protease fraction
 
Creator PATHAK, CHARU
GHOURI, S K
KUMAR, S K
SAXENA, M M
 
Subject Cysteine proteases, Haemonchus contortus, Small ruminants, Vaccine
 
Description Haemonchus contortus is an economically important, highly pathogenic blood-sucking nematode parasite of ruminants. A study was conducted to characterize the cathepsin B-like cysteine protease gene of H. contortus of Indian origin, which has been explored as potential target for vaccine development. Adult H. contortus worms were collected from the abomasum of sheep and goats and whole genomic DNA was extracted and amplified using appropriate primers. The expected amplicon of 767 bp was cloned into T-vector using GeNeiTM Instant cloning kit. Immunization study was conducted to examine the effect of cysteine protease fraction on the faecal egg counts in lambs. It was found that the egg per gram of faeces was lower in immunized group as compared to control group (at 10, 11, 12, and 15 week). At post mortem, the worms recovered from the abomasa were lower in immunized group (800.40±101.8) as compared to control group (950.93±300.29). The results indicated that the immunization with cysteine protease fraction in lambs reduces the faecal egg count, but did not remove all the worms from lambs.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2015-11-06
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/53042
 
Source The Indian Journal of Animal Sciences; Vol 85, No 11 (2015)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/53042/22346
 
Rights Copyright (c) 2015 The Indian Journal of Animal Sciences