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Synergistic effects of insulin-like growth factor ii (IGF-II) and leutinizing hormone (LH) on steroid hormone secretion by cultured bovine granulosa cells

Indian Agricultural Research Journals

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Title Synergistic effects of insulin-like growth factor ii (IGF-II) and leutinizing hormone (LH) on steroid hormone secretion by cultured bovine granulosa cells
 
Creator SMITH, O F
OGUNSOLA, A A
LADOKUN, A O
R, A AJADI
 
Subject Bovine granulosa cells; IGF-II; LH
 
Description The synergistic effects of insulin-like growth factor II (IGF-II) and leutinizing hormone (LH) and the effect of IGF-II alone on production of progesterone (P₄) and estradiol (E₂) by bovine granulosa cells (GC) cultured in vitro were determined in this study. Bovine granulosa cells obtained from ovaries of slaughtered cows were cultured for 4 days in carbon (IV) dioxide (CO₂) incubator at 37°C, 5% CO₂ in atmospheric air and 100% relative humidity, using tissue culture medium199 (TCM 199) as culture medium. The medium was changed on the second day and testosterone added to serve as substrate for estadiol production. Insulin growth factor II (IGF-II) alone, or IGF-II + LH were added into the culture medium at 0, 0.1, 1.0, 10, 50, and 100 (ng/ml) levels of inclusion. Concentrations of progesterone and estradiol 17β produced were measured by radioimmunoassay. The results obtained showed that IGF-II alone and IGF-II + LH had significant effects on progesterone and estradiol produced by cultured granulosa cells. The result also revealed higher values of progesterone (4.78 ng/ml) and estradiol 17β (9.68 pg/ml) when IGF-II (10 ng/ml) and LH (50 ng/ml) were included together in the culture medium as compared with progesterone value (1.84 ng/ml) and estradiol 17β value (3.57 pg/ml) when IGF-II alone was included in TCM-199. From the result obtained, it can be concluded that IGF-II (10 ng/ml) + LH (50ng/ml) could be added to TCM-199 to give better synergistic effects on bovine granulosa progesterone and estradiol production as compared to addition of IGF-II (50ng/ml) alone.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-11-14
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/12662
 
Source The Indian Journal of Animal Sciences; Vol 81, No 11 (2011)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/12662/6335
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences