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Influence of storage period on motion characteristics of frozen-thawed Awassi ram spermatozoa

Indian Agricultural Research Journals

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Title Influence of storage period on motion characteristics of frozen-thawed Awassi ram spermatozoa
 
Creator Joshi, Anil
Bag, Sadhan
Rawat, P S
Mittal, J P
 
Subject Awassi; Computer-aided semen analysis (CASA); Cryopreservation; Semen; Sheep; Storage
 
Description The motion characteristics of Awassi ram semen, cryopreserved in 0.25 ml straws, Were studied after 8, 16 or 24 months of storage by computer-assisted semen analysis technique. Ejaculates of good quality were extended @ 1 000 million spermatozoa/ ml and frozen @ -250°C / min in a cell freezer. Frozen straws were plunged in liquid nitrogen and thawed at 50° C for 10 sec in a water bath. The mean post-thaw recovery of motile spermatozoa was >70% at the 3 storage periods varying from 72.0 to 74.7%. Storage had significant effect on per cent medium but had no significant effect on per cent motility, per cent rapid, per cent slow, per cent linearity, per cent straightness, curvilinear velocity, average path velocity, straight line velocity, beat frequency and amplitude of lateral head displacement of spermatozoa. Post-thaw viability of ram spermatozoa remained unaltered during long-term preservation in mini-straws.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-07-25
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/22309
 
Source The Indian Journal of Animal Sciences; Vol 68, No 10 (1998)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/22309/10967
 
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