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Restriction endonuclease analysis of genomic DNA of isolates of Trypanosoma evansi

Indian Agricultural Research Journals

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Title Restriction endonuclease analysis of genomic DNA of isolates of Trypanosoma evansi
 
Creator YADAV, S N
GHORUI, S K
RAY, D
 
Subject DNNgenome, Restriction analysis, Trypanosoma evansi
 
Description The present study was initiated to focus on the differences or similarities among different isolates of Trypanosoma evansi through restriction endonuclease profile. The genomic DNA of T.evansi, isolated from naturally infected buffalo, horse and camel were analysed. A panel of restriction enzymes-Alu I, Dra I, EcoR I, Hind III, Kpn I, Not I, Pst I, Sal I, Sma I and Taq I were used for complete digestion of genomic DNA. The agarose gel electrophoresis of digested DNA samples appeared as continuous smear along the electrophoretic tracks on ethidium bromide staining revealing the complex size oftrypanosome genome. There was no fixed restriction site, but in restriction enzyme Dra I and Alu I where restriction site at the region of 1.5 kb and 100 bp, respectively, appeared with background smear of DNA fragments. No heterogeneity in the nuclear DNA restriction endonuclease profile among the isolates was recorded.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2014-03-06
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/38555
 
Source The Indian Journal of Animal Sciences; Vol 74, No 5 (2004)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/38555/17329
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences