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Phylogenetic analysis of classical swine fever virus (CSFV) by cloning and sequencing of partial 5' non-translated genomic region

Indian Agricultural Research Journals

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Title Phylogenetic analysis of classical swine fever virus (CSFV) by cloning and sequencing of partial 5' non-translated genomic region
 
Creator SINGH, V K
SAIKUMAR, G
BANDYOPADHYAY, S K
PALIWAL, O P
 
Subject Classical swine fever virus, Genetic grouping, Phylogenetic analysis, 5' NTR
 
Description Classical swine fever (CSF) is an economically important and highly contagious viral disease of pigs across the world. Despite its office International des Epizooties (OIE) list A status, the disease has not been studied extensively in India and epidemilogical and phylogenetic information with regards to CSFV is lacking. In the present study, an attempt was made towards phylogenetic characterization of virulent (CSFV/MP) and lapinized vaccine (CSF/LAP) strains of CSFV. Reverse transcription-polymerase chain raction (RT-PCR) amplified products from 5'non-translated region (NTR) of both the strains were cloned and sequenced. The truncated genomic sequences were analyzed and compared with published CSFV sequences using LASERGENE and PHYLIP softwares. Phylogenetic analysis resulted into placement of CSFV/MP and CSFV/LAP strains into genorgroup 1.1. The present study provides phylogenetic information and paves way for large scale molecular epidemological studies with respect to CSF in India.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2014-04-16
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/39392
 
Source The Indian Journal of Animal Sciences; Vol 74, No 11 (2004)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/39392/17694
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences