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Molecular characterization of India isolates of Pasteurella multocida serotype B:2 using REA, RAPD-PCR and ribotyping

Indian Agricultural Research Journals

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Title Molecular characterization of India isolates of Pasteurella multocida serotype B:2 using REA, RAPD-PCR and ribotyping
 
Creator DUTTA, T K
GAUTAM, RAJEEV
SINGH, V P
KUMAR, A A
 
Subject Pasteurella multocida, B:2, India
 
Description Pasteurella multocida (serotype B:2) isolates (35) from different animals were tested for differentiation by restriction endonuclease analysis (REA), ribotyping and randomly amplified polymorphic-DNC (RAPD)-PCR analysis. Reproducible patterns were obtained from all the 3 methods. All the isolates were found similar phenotypically, as well as genotypically irrespective of their hosts and genographical locations. All the strains were also similar with the standard vaccine strain (P52 Izatnagar) used in India. Two specific bands were observed at 8.4-71 kb range having diagostic value for B:2 sertype when digests with enzyme Hha I, which were absent in other serotypes.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2014-03-21
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/38844
 
Source The Indian Journal of Animal Sciences; Vol 74, No 6 (2004)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/38844/17466
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences