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A rapid method for peR amplification of specific genes of Mycobacterium avium sub sp. paratuberculosis

Indian Agricultural Research Journals

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Title A rapid method for peR amplification of specific genes of Mycobacterium avium sub sp. paratuberculosis
 
Creator VIJAYARANI, K
KUMAMAN, K
 
Subject Mycobacterium avium sub sp. paratuberculosis; Johne's disease; Paratuberculosis; Polymerase chain reaction
 
Description The present study describes a simple and rapid method for amplifying various genes of Mycobacterium aviLl1n subsp. paratuberculosis (MAP) by polymerase chain reaction. Culture supernatants of MAP, when used as template resulted in the amplification of IS 900, ISMav2, ISMap02, and superoxide dismutase genes of MAP. This Inethod circumvents the specific isolation of genomic DNA frOln concentrated MAP organisms for ainplifying MAP genes and saves valuable reagents and time. 
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-03-14
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4873
 
Source The Indian Journal of Animal Sciences; Vol 78, No 10 (2008)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4873/2040
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences