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Evaluation of ELISA and IHA for the detection of agglutinins in goats vaccinated with an experimental killed Mycoplasma mycoides subsp capri saponin vaccine

Indian Agricultural Research Journals

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Title Evaluation of ELISA and IHA for the detection of agglutinins in goats vaccinated with an experimental killed Mycoplasma mycoides subsp capri saponin vaccine
 
Creator RANA, RAJNEESH
SRIVASTAVNA, N C
SINGH, V P
SRIVASTAVA, S K
 
Subject Agglutinin, ELISA, IHA, Mycoplasma mycoides subsp capri, Vaccine
 
Description An indirect ELISA was used for the first time to detect Mycoplasma capri antibodies in goats and was compared withindirect haemagglutinatioll test. Goats (36) under 1 year of age and of either sex (negative for M.capri antibodies) were used in 3 groups of 17,17 and 2. Two different freeze-dried M.capri saponin vaccines i.e. vaccine A (protein concentration 1.0 mg/dose) and vaccine B (protein concentration 1.5 mg dose) were prepared and inoculated in 2 different group of goats (17 animals in each group). Indirect haemagglutination test could detect early antibodies whereas; ELISA could detect both early and late antibodies. Enzyme linked immunosorbent assay was adjudged to be comparatively superior for the detection of M.capri agglutinins.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2014-03-27
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/39080
 
Source The Indian Journal of Animal Sciences; Vol 74, No 8 (2004)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/39080/17588
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences