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Molecular detection and characterization of Indian isolates of Pasteurella multocida serogroup ‘D’

Indian Agricultural Research Journals

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Title Molecular detection and characterization of Indian isolates of Pasteurella multocida serogroup ‘D’
 
Creator DUTTA, T K
SINGH, V P
KUMAR, A A
 
Subject Pasteurella; PCR; REA; Serogroup D
 
Description Five Pasteurella multocida serogroup D isolated from pig (n=4, serotype D: 1) and sheep (n=1, serotype D: 3) were tested for molecular identification and characterization. All the isolates were detected by species specific PCR (PMPCR) and ~460 bp products were obtained. RE analysis of the isolates using enzymes Hha I and Hpa II generated very clear and readable discriminatory banding profiles and 3 different RE profiles obtained out of 4 P. multocida isolates of serotype D: 1. Among the 2 enzymes used in this study, Hpa I had better discriminatory power than Hha I. Single primer based RAPD-PCR assay generated readable and distinct banding patterns between serotypes, but failed to differentiate 4 isolates of P. multocida serotype D: 1. Molecular detection using species specific PCR and characterization by molecular techniques like REA and RAPD-PCR could enable typing of P. multocida strains as a complement of classical capsular and somatic typing in preliminary characterization. 
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-03-23
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/5096
 
Source The Indian Journal of Animal Sciences; Vol 79, No 11 (2009)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/5096/2140
 
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