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Difficidin class of polyketide antibiotics from marine macroalga-associated Bacillus as promising antibacterial agents

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Field Value
 
Relation http://eprints.cmfri.org.in/15233/
https://link.springer.com/article/10.1007/s00253-021-11390-z
https://doi.org/10.1007/s00253-021-11390-z
 
Title Difficidin class of polyketide antibiotics from marine
macroalga-associated Bacillus as promising antibacterial agents
 
Creator Chakraborty, Kajal
Vinaya, K K
Joy, Minju
Dhara, Subhajit
 
Subject Bioactive compounds
Biochemistry
Algae
 
Description A heterotrophoic Bacillus amyloliquefaciens MTCC12713 isolated from an intertidal macroalga Kappaphycus alverezii displayed promising antibacterial activities against multidrug-resistant bacteria. Genome mining of the bacterium predicted biosynthetic gene clusters coding for antibacterial secondary metabolites. Twenty-one membered macrocyclic lactones, identified as difficidin analogues bearing 6-hydroxy-8-propyl carboxylate, 9-methyl-19-propyl dicarboxylate, 6-methyl-9-propyl dicarboxylate-19-propanone, and (20-acetyl)-6-methyl-9-isopentyl dicarboxylate (compounds 1 through 4) functionalities were purified through bioassay-guided fractionation. The difficidin analogues exhibited bactericidal activities against vancomycin-resistant Enterococcus faecalis, methicillin-resistant Staphylococcus aureus, and other drug-resistant strains, such of Klebsiella pneumonia and Pseudomonas aeruginosa with the minimum inhibitory concentration of about 2–9 × 10−3 μM. A plausible enzyme-catalyzed biosynthetic pathway that is generated through addition of acrylyl initiator unit by repetitive decarboxylative Claisen condensation modules with malonate units was recognized, and their structures were corroborated with gene organization of the dif operon, which could comprehend dif A-O (~ 70 kb). Drug-likeness score for 5-ethoxy-28-methyl-(9-methyl-19-propyl dicarboxylate) difficidin (compound 2, 0.35) was greater than those of other difficidin analogues, which corroborated the potential in vitro antibacterial properties of the former. The present study demonstrated the potential of difficidin analogues for pharmaceutical and biotechnological uses against the bottleneck of emergent drug-resistant pathogens.
 
Publisher Springer
 
Date 2021
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/15233/1/Applied%20Microbiology%20and%20Biotechnology_2021Kajal%20Chakraborty_Difficidin_class_of_polyketide_antibiotics.pdf
Chakraborty, Kajal and Vinaya, K K and Joy, Minju and Dhara, Subhajit (2021) Difficidin class of polyketide antibiotics from marine macroalga-associated Bacillus as promising antibacterial agents. Applied Microbiology and Biotechnology, 105. pp. 6395-6408.