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Development of monoclonal antibodies against bovine herpesvirus-1 (BHV-1)

Indian Agricultural Research Journals

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Title Development of monoclonal antibodies against bovine herpesvirus-1 (BHV-1)
 
Creator Kaur, Gurpreet
Dwivedi, P N
Ramneek, Ramneek
Maiti, N K
 
Subject AGPT; Bovine Herpesvirus-1; ELISA; Hybridoma cell culture; Monoclonal antibody.
 
Description The BHV-1 antigen was produced in bulk by propagating BHV-1 in Madin Darby bovine kidney cell line. The antigen purified by 20–40% sucrose density gradient centrifugation was confirmed for presence of virus. The BALB/C mice were immunized by purified virus via intraperitoneal route. The spleenic lymphocytes from immunized mice were fused with myeloma cells using polyethylene glycol as a fusion agent and suspended in HAT (hypoxanthine aminopterin thymidine) medium for differentiation of hybrids. A total of 11 clones were observed, out of which 7 were positive for antibody production. The supernatant of all the positive clones showed very low concentration of antibody, except the clone B₆.₁, where the concentration of antibody was higher than other positive clones. The culture supernatant of B₆.₁ hybrid clone was isotyped by enzyme linked immunosorbent assay (ELISA) based isotyping kit and isotype of monoclonal antibody was of IgM class. In western blotting, the IgM monoclonal antibody identified the 27 KD protein of BHV-1.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-07-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/21744
 
Source The Indian Journal of Animal Sciences; Vol 82, No 7 (2012)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/21744/10856
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences