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Hepatic microsporidiosis of mudskipper, Boleophthalmus dussumieri Valenciennes, 1837 (Perciformes: Gobiidae), due to Microgemma sp.

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Relation http://eprints.cmfri.org.in/15434/
https://link.springer.com/article/10.1007/s12639-021-01419-5
10.21203/rs.3.rs-344161/v1
 
Title Hepatic microsporidiosis of mudskipper, Boleophthalmus
dussumieri Valenciennes, 1837 (Perciformes: Gobiidae), due
to Microgemma sp.
 
Creator Vandana, V R
Poojary, Nalini
Tripathi, Gayatri
Pavan Kumar, Annam
Sanil, N K
Rajendran, K V
 
Subject Fish Disease
Fish biology
Invertebrata
 
Description The present study reports a case of hepatic
microsporidiosis caused by Microgemma sp. in brackishwater
fish, Boleophthalmus dussumieri (Valenciennes, 1837)
(n = 60), from the west coast of India. An eight-month study
from September 2017 to April 2018 revealed a prevalence of
11.7% for this parasite. The microsporidian showed tissuespecific infection and did not reveal any gross pathology in
infected fish. Small whitish cysts containing microspores of
size 0.3–0.5 mm were observed in the liver of fish. The range
of pyriform microsporidian spore size varied from
2.9–3.77 9 1.85–2.67 lm. Scanning electron microscopy of
the spores showed a distinct groove on the anterior end of the
spore for polar tube extrusion. Polymerase chain reaction
(PCR) amplification of the DNA extracted from the
microsporidian-infected liver tissue using primers targeting
small ribosomal subunit DNA (SSU rDNA) yielded
* 1340 bp amplicon and the genetic distance analysis
showed a 0.2% variation with the reported M. tilanpasiri.
Accordingly, in the phylogenetic tree, the present species of
Microgemma clustered with M. tilanpasiri. Even though, the
morphomeristic characters of the present Microgemma sp.
was marginally different from the reported M. tilanpsasiri;
the SSU rDNA showed considerably higher similarity with
M. tilanpasiri. Thus, we report the species of Microgemma as
Microgemma aff. tilanpasiri from a new host. This is the first
report of a microsporidian from B. dussumieri and the first
record of the genus Microgemma from India
 
Publisher Springer
 
Date 2021-03-08
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/15434/2/Journal%20of%20Parasitic%20Diseases_2021_Sanil%20N%20K.pdf
Vandana, V R and Poojary, Nalini and Tripathi, Gayatri and Pavan Kumar, Annam and Sanil, N K and Rajendran, K V (2021) Hepatic microsporidiosis of mudskipper, Boleophthalmus dussumieri Valenciennes, 1837 (Perciformes: Gobiidae), due to Microgemma sp. Journal of Parasitic Diseases. pp. 1-8.