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Culture of spermatogonial stem cells and use of surrogate sires as a breeding technology to propagate superior genetics in livestock production: A systematic review

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Title Culture of spermatogonial stem cells and use of surrogate sires as a breeding technology to propagate superior genetics in livestock production: A systematic review
 
Creator Nakami, W.
Kipyegon, A.N.
Nguhiu-Mwangi, J.
Tiambo, Christian K.
Kemp, Stephen J.
 
Subject livestock
animal breeding
genetics
animal production
 
Description Background and Aim: Spermatogonial stem cells (SSCs) have previously been isolated from animals' testes, cultured in vitro, and successfully transplanted into compatible recipients. The SSC unique characteristic has potential for exploitation as a reproductive tool and this can be achieved through SSC intratesticular transplantation to surrogate sires. Here, we aimed at comprehensively analyzing published data on in vitro maintenance of SSC isolated from the testes of livestock animals and their applications. Materials and Methods: The literature search was performed in PubMed, Science Direct, and Google Scholar electronic databases. Data screening was conducted using Rayyan Intelligent Systematic Review software (https://www.rayyan.ai/). Duplicate papers were excluded from the study. Abstracts were read and relevant full papers were reviewed for data extraction. Results: From a total of 4786 full papers screened, data were extracted from 93 relevant papers. Of these, eight papers reported on long-term culture conditions (>1 month) for SSC in different livestock species, 22 papers on short-term cultures (5-15 days), 10 papers on transfection protocols, 18 papers on transplantation using different methods of preparation of livestock recipients, and five papers on donor-derived spermatogenesis. Conclusion: Optimization of SSC long-term culture systems has renewed the possibilities of utilization of these cells in gene-editing technologies to develop transgenic animals. Further, the development of genetically deficient recipients in the endogenous germline layer lends to a future possibility for the utilization of germ cell transplantation in livestock systems.
 
Date 2021-12-31
2022-01-04T13:22:19Z
2022-01-04T13:22:19Z
 
Type Journal Article
 
Identifier Nakami, W., Kipyegon, A.N., Nguhiu-Mwangi, J., Tiambo, C. and Kemp, S. 2021. Culture of spermatogonial stem cells and use of surrogate sires as a breeding technology to propagate superior genetics in livestock production: A systematic review. Veterinary World 14(12):3235-3248.
2231-0916
https://hdl.handle.net/10568/117350
https://doi.org/10.14202/vetworld.2021.3235-3248
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format p. 3235-3248
 
Publisher Veterinary World
 
Source Veterinary World