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Cryopreservation of Abies alba embryogenic tissues by slow-freezing method

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Title Cryopreservation of Abies alba embryogenic tissues by slow-freezing method
 
Creator Salaj, Terezia
Panis, Bartholomeus
Klubicova, Katarina
Salaj, Jan
 
Subject Pinophyta
cryopreservation
regeneration
embryonic development
criopreservación
regeneración
desarrollo embrionario
 
Description Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing approach. Four cell lines were incubated for 24 h on a medium with 0.5 M sorbitol and pre-treated with 5% DMSO. Subsequently,
the tissues were frozen at a cooling rate of 1 °C min-1 to -40 °C and transferred to liquid nitrogen for 72 hours. After thawing in a water bath at 40 °C, the tissues were cultivated on a proliferation medium. All tested lines
recovered, but variations in regrowth frequencies across cell lines were noticed (91.66 to 100%). The recovered tissues showed similar features to the control 2 (non-pre-treated and non-cryopreserved tissues). In the
accumulation of fresh and dry mass, no statistically significant differences were observed between cryopreserved cultures and control 2. The cryopreserved tissues produced cotyledonary somatic embryos capable of
germination. Microscopic observations revealed considerable structural changes as a consequence of the cryopreservation procedure. The long vacuolated suspensor cells were disrupted, and mostly the meristematic
cells of the embryonal region survived. The typical bipolar structure of early somatic embryos has been regained during the post-thaw period. Differences in cryotolerance across cell lines were also observed.
 
Date 2022-12-06
2022-12-29T10:41:49Z
2022-12-29T10:41:49Z
 
Type Journal Article
 
Identifier Salaj, T.; Panis, B.; Klubicova, K.; Salaj, J. (2022) Cryopreservation of Abies alba embryogenic tissues by slow-freezing method. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 50(4) : 12770 15 p. ISSN: 0255-965X
0255-965X
https://hdl.handle.net/10568/126396
https://doi.org/10.15835/nbha50412770
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 15 p.
application/pdf
 
Publisher Academic Journals
 
Source Notulae Botanicae Horti Agrobotanici Cluj-Napoca