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Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana

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Title Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana
 
Creator Alkan, Mehtap
Bayraktar, Harun
Imren, Mustafa
Ozdemir, Fatih
Lahlali, Rachid
Mokrini, Fouad
Paulitz, Timothy
Dababat, Abdelfattah A.
Özer, Göksel
 
Subject diseases
gene expression
pathogens
wheat
Bipolaris sorokiniana
 
Description Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat worldwide. This study investigated the aggressiveness of B. sorokiniana isolates from different wheat-growing areas of Bolu province in Turkey on the cultivar Seri-82. Host susceptibility of 55 wheat cultivars was evaluated against the most aggressive isolate. Our results indicated that the cultivars Anafarta and Koç-2015 were the most resistant. A specific and sensitive qPCR assay was developed for detecting the pathogen in plant tissues and evaluating wheat plants with different resistance levels. Three primer sets, BsGAPDHF/BsGAPDHR, BsITSF/BsITSR, and BsSSUF/BsSSUR, were designed based on glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacers, and 18S rRNA loci of B. sorokiniana with detection limits of 1, 0.1, and 0.1 pg of pathogen DNA, respectively. The qPCR assay was highly sensitive and did not amplify DNA from the other closely related fungal species and host plants. The protocol differentiated wheat plants with varying degrees of resistance. The assay developed a useful tool for the quantification of the pathogen in the early stages of infection and may provide a significant contribution to a more efficient selection of wheat genotypes in breeding studies. In the present study, expression levels of PR proteins, phenylalanine ammonia-lyase, catalase, ascorbate peroxidase, and superoxide dismutase enzymes were upregulated in Anafarta (resistant) and Nenehatun (susceptible) cultivars at different post-infection time points, but more induced in the susceptible cultivar. The results showed considerable variation in the expression levels and timing of defense genes in both cultivars.
 
Date 2022
2023-01-03T13:17:36Z
2023-01-03T13:17:36Z
 
Type Journal Article
 
Identifier Alkan, M., Bayraktar, H., İmren, M., Özdemir, F., Lahlali, R., Mokrini, F., Paulitz, T., Dababat, A. A., & Özer, G. (2022). Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana. Journal of Fungi, 8(2), 149. https://hdl.handle.net/10883/22233
2309-608X
https://hdl.handle.net/10568/126497
https://hdl.handle.net/10883/22233
https://doi.org/10.3390/jof8020149
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format application/pdf
 
Publisher MDPI
 
Source Journal of Fungi