Spent pleurotus ostreatus substrate has potential for managing Fusarium Wilt of Banana
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Title |
Spent pleurotus ostreatus substrate has potential for managing Fusarium Wilt of Banana
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Creator |
Ocimati, Walter
Were, Evans M. Tazuba, Anthony Fredrick Kadama Dita Rodriguez, Miguel Angel Zheng, Si-Jun Blomme, Guy |
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Subject |
basidiomycota
biological control pathogens metabolites fusarium oxysporum control biológico organismos patógenos |
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Description |
A range of basidiomycetes including the edible mushroom Pleurotus ostreatus (Po) can suppress plant pathogens such as Fusarium spp. With the current increase in production and consumption of Po in Uganda, the spent Po substrate (SPoS) could be an alternative to manage Fusarium wilt of banana (FWB), caused by the soil borne pathogen Fusarium oxysporum f. sp. cubense, race 1 (Foc). This study determined the potential of SPoS to inhibit Foc in vitro and in potted plants. In vitro studies confirmed suppression of Foc in pure co-culture (Po vs. Foc) assays and media amended with different concentrations (0% to 50% w/v) of un-sterilized SPoS filtrates. Foc growth in the sterile SPoS filtrate was comparable to the water control, suggesting possible roles of biotic or thermolabile components of the SPoS. To further verify the suppressive effects of SPoS, pot experiments were carried out with a resistant (‘Mbwazirume’, AAA) and susceptible (‘Sukali Ndizi’, AAB) banana cultivar using both artificially and naturally infested soils. Independent of the inoculation method, SPoS significantly reduced the severity of FWB in pot experiments. Susceptible cultivar ‘Sukali Ndizi’ growing in substrates amended with SPoS showed lower (1.25) corm damage (Scale 0–5) than the un-amended control (3.75). No corm damage was observed in uninoculated controls. The resistant cultivar ‘Mbwazirume’, showed slight (0.25) corm damage only in the Foc-inoculated plants without SPoS. These findings suggest that SPoS could be used as part of the management practices to reduce the impact of FWB. |
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Date |
2021-11-09
2021-12-03T15:02:07Z 2021-12-03T15:02:07Z |
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Type |
Journal Article
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Identifier |
Ocimati, W.; Were, E.; Tazuba, A.F.; Dita, M.; Zheng, S.-J.; Blomme, G. (2021) Spent pleurotus ostreatus substrate has potential for managing Fusarium Wilt of Banana. Journal of Fungi 7(11): 946. ISSN: 2309-608X
2309-608X https://hdl.handle.net/10568/116504 https://doi.org/10.3390/jof7110946 |
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Language |
en
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Rights |
CC-BY-4.0
Open Access |
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Format |
application/pdf
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Publisher |
MDPI AG
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Source |
Journal of Fungi
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