Record Details

Lack of association between fluconazole susceptibility and ERG11 nucleotide polymorphisms in Cryptococcus neoformans clinical isolates from Uganda

CGSpace

View Archive Info
 
 
Field Value
 
Title Lack of association between fluconazole susceptibility and ERG11 nucleotide polymorphisms in Cryptococcus neoformans clinical isolates from Uganda
 
Creator Atim, Priscilla Belbir
Meya, David B.
Gerlach, Elliot S.
Muhanguzi, Dennis
Male, Allan
Kanamwanji, Benedict
Nielsen, Kirsten
 
Description Fluconazole is the drug of choice for cryptococcal meningitis (CM) monoprophylaxis in resource-limited settings such as Uganda. Emerging fluconazole resistance linked to mutations in the Cryptococcus neoformans ERG11 gene (CYP51) has been observed in clinical isolates. Currently, the single nucleotide polymorphisms [SNPs] in the Cryptococcus spp. ERG11 gene that could be responsible for fluconazole resistance are poorly characterized within Ugandan C. neoformans clinical isolates. If available, this information would be useful in the management of cryptococcosis among HIV patients. This cross-sectional study investigates the SNPs present in the coding region of the C. neoformans ERG11 gene to determine the relationship between the SNPs identified and fluconazole susceptibility of the clinical isolates. 310 C. neoformans isolates recovered from the Cerebrospinal Fluid (CSF) of patients with HIV and cryptococcal meningitis were examined. The fluconazole half-maximal inhibitory concentrations (IC50 range: 0.25–32 μg/mL) was determined using the microbroth dilution method. A total of 56.1% of the isolates had low IC50 values of
 
Date 2022-05-10
2022-12-05T14:43:23Z
2022-12-05T14:43:23Z
 
Type Journal Article
 
Identifier Atim, P.B.; Meya, D.B.; Gerlach, E.S.; Muhanguzi, D.; Male, A.; Kanamwanji, B.; Nielsen, K. (2022) Lack of association between fluconazole susceptibility and ERG11 nucleotide polymorphisms in Cryptococcus neoformans clinical isolates from Uganda. Journal of Fungi 8(5):508. ISSN: 2309-608X
2309-608X
https://hdl.handle.net/10568/125776
https://doi.org/10.3390/jof8050508
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 508
application/pdf
 
Publisher MDPI
 
Source Journal of Fungi