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Chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the Western Amazon region of Colombia

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Title Chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the Western Amazon region of Colombia
 
Creator Suárez, Juan Carlos
Vanegas, José Iván
Contreras, Amara Tatiana
Anzola, José Alexander
Urban, Milan O.
Beebe, Stephen E.
Rao, Idupulapati M.
 
Subject acid soils
agronomic characters
performance assessment
disease resistance
dry matter
yields
heat-high temperature
common beans
phaseolus
 
Description The evaluation of disease resistance is considered an important aspect of phenotyping for crop improvement. Identification of advanced lines of the common bean with disease resistance contributes to improved grain yields. This study aimed to determine the response of the photosyn- thetic apparatus to natural pathogen infection by using chlorophyll (Chla) fluorescence parameters and their relationship to the agronomic performance of 59 common bean lines and comparing the photosynthetic responses of naturally infected vs. healthy leaves. The study was conducted over two seasons under acid soil and high temperature conditions in the western Amazon region of Colombia. A disease susceptibility index (DSI) was developed and validated using chlorophyll a (Chla) fluorescence as a tool to identify Mesoamerican and Andean lines of common bean (Phaseolus vulgaris L.) that are resistant to pathogens. A negative effect on the functional status of the photo- synthetic apparatus was found with the presence of pathogen infection, a situation that allowed the identification of four typologies based on the DSI values ((i) moderately resistant; (ii) moderately susceptible; (iii) susceptible; and (iv) highly susceptible). Moderately resistant lines, five of them from the Mesoamerican gene pool (ALB 350, SMC 200, BFS 10, SER 16, SMN 27) and one from the Andean gene pool (DAB 295), allocated a higher proportion of energy to photochemical processes, which increased the rate of electron transfer resulting in a lower sensitivity to disease stress. This photosynthetic response was associated with lower values of DSI, which translated into an increase in the accumulation of dry matter accumulation in different plant organs (leaves, stem, pods and roots). Thus, DSI values based on chlorophyll fluorescence response to pathogen infection could serve as a phenotyping tool for evaluating advanced common bean lines. Six common bean lines (ALB 350, BFS 10, DAB 295, SER 16, SMC 200 and SMN 27) were identified as less sensitive to disease stress under field conditions in the western Amazon region of Colombia, and these could serve as useful parents for improving the common bean for multiple stress resistance.
 
Date 2022-05-21
2023-01-13T10:09:42Z
2023-01-13T10:09:42Z
 
Type Journal Article
 
Identifier Suárez, J.C.; Vanegas, J.I.; Contreras, A.T.; Anzola, J.A.; Urban, M.O.; Beebe, S.E.; Rao, I.M. (2022) Chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the Western Amazon region of Colombia. Plants 11(10):1371. ISSN: 2223-7747
2223-7747
https://hdl.handle.net/10568/127036
https://doi.org/10.3390/plants11101371
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 1371
application/pdf
 
Publisher MDPI AG
 
Source Plants