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SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis

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Title SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis
 
Creator Zou, Xiaoyan
Huang, Rui
Wang, Linjie
Wang, Guihua
Miao, Ye
Rao, Idupulapati M.
Liu, Guodao
Chen, Zhijian
 
Subject homeostasis
gene expression
stylosanthes guianensis
trace elements
pcr
 
Description Transporters belonging to the natural resistance-associated macrophage protein (Nramp) family play important roles in metal uptake and homeostasis. Although Nramp members have been functionally characterized in plants, the role of Nramp in the important tropical forage legume Stylosanthes guianensis (stylo) is largely unknown. This study aimed to determine the responses of Nramp genes to metal stresses and investigate its metal transport activity in stylo. Five SgNramp genes were identified from stylo. Expression analysis showed that SgNramp genes exhibited tissue preferential expressions and diverse responses to metal stresses, especially for manganese (Mn), suggesting the involvement of SgNramps in the response of stylo to metal stresses. Of the five SgNramps, SgNramp1 displayed the highest expression in stylo roots. A close correlation between SgNramp1 expression and root Mn concentration was observed among nine stylo cultivars under Mn limited condition. The higher expression of SgNramp1 was correlated with a high Mn uptake in stylo. Subsequent subcellular localization analysis showed that SgNramp1 was localized to the plasma membrane. Furthermore, heterologous expression of SgNramp1 complemented the phenotype of the Mn uptake-defective yeast (Saccharomyces cerevisiae) mutant Dsmf1. Mn concentration in the yeast cells expressing SgNramp1 was higher than that of the empty vector control, suggesting the transport activity of SgNramp1 for Mn in yeast. Taken together, this study reveals that SgNramp1 is a plasma membrane–localized transporter responsible for Mn uptake in stylo.
 
Date 2022-10-06
2023-01-14T18:53:02Z
2023-01-14T18:53:02Z
 
Type Journal Article
 
Identifier Zou, X.; Huang, R.; Wang, L.; Wang, G.; Miao, Y.; Rao, I.; Liu, G.; Chen, Z. (2022) SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis. Frontiers in Plant Science 13:1027551. ISSN: 1664-462X
1664-462X
https://hdl.handle.net/10568/127145
https://doi.org/10.3389/fpls.2022.1027551
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format application/pdf
 
Publisher Frontiers Media SA
 
Source Frontiers in Plant Science