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Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea

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Title Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea
 
Creator Alo, Fida
Ran, Anupalli
Baum, Michael
Singh, Sarvjeet
Kehel, Zakaria
Rani, Upasana
Udupa, Sripada M.
El-Shamaa, Khaled
Alsamman, Alsamman
Istanbuli, Tawffiq
Attar, Basem
Hamwieh, Aladdin
Amri, Ahmed
 
Subject goal 2 zero hunger
Chickpea
ascochyta blight
resistance
quantitative trait loci (qtls)
chickpea
nutrition, health and food security
genotyping-by-sequencing (gbs)
 
Description Ascochyta blight (AB), caused by the fungal pathogen Ascochyta rabiei, is a devastating foliar disease of chickpea (Cicer arietinum L.). The genotyping-by-sequencing (GBS)-based approach was deployed for mapping QTLs associated with AB resistance in chickpea in two recombinant inbred line populations derived from two crosses (AB3279 derived from ILC 1929 × ILC 3279 and AB482 derived from ILC 1929 × ILC 482) and tested in six different environments. Twenty-one different genomic regions linked to AB resistance were identified in regions CalG02 and CalG04 in both populations AB3279 and AB482. These regions contain 1,118 SNPs significantly associated with AB resistance (p ≤ 0.001), which explained 11.2–39.3% of the phenotypic variation (PVE). Nine of the AB resistance-associated genomic regions were newly detected in this study, while twelve regions were known from previous AB studies. The proposed physical map narrows down AB resistance to consistent genomic regions identified across different environments. Gene ontology (GO) assigned these QTLs to 319 genes, many of which were associated with stress and disease resistance, and with most important genes belonging to resistance gene families such as leucine-rich repeat (LRR) and transcription factor families. Our results indicate that the flowering-associated gene GIGANTEA is a possible key factor in AB resistance in chickpea. The results have identified AB resistance-associated regions on the physical genetic map of chickpea and allowed for the identification of associated markers that will help in breeding of AB-resistant varieties.
 
Date 2022-04-25
2022-12-02T16:59:18Z
2022-12-02T16:59:18Z
 
Type Journal Article
 
Identifier Fida Alo, Anupalli Ran, Michael Baum, Sarvjeet Singh, Zakaria Kehel, Upasana Rani, Sripada M. Udupa, Khaled El-Shamaa, Alsamman Alsamman, Tawffiq Istanbuli, Basem Attar, Aladdin Hamwieh, Ahmed Amri. (25/4/2022). Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea. Frontiers in Plant Science, 13.
1664-462X
https://hdl.handle.net/10568/125765
https://doi.org/10.5281/zenodo.5236818
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format application/pdf
 
Publisher Frontiers Media
 
Source Frontiers in Plant Science