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Genetic characterization of oleaginous bottle gourd (Lagenaria siceraria) germplasm from Côte d'Ivoire using agromorphological and molecular markers

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Title Genetic characterization of oleaginous bottle gourd (Lagenaria siceraria) germplasm from Côte d'Ivoire using agromorphological and molecular markers
 
Creator Gbotto, A.A.
Yao, N.K.
Kitavi, M.
Osama, S.K.
Habimana, R.
Koffi, K.K.
Zoro Bi, I.A.
 
Subject core collections
genetic variation
Lagenaria siceraria
germplasm
 
Description Being difficult to regenerate and maintain the seeds, the oleaginous bottle gourd was investigated using nine agromorphological traits and 31 amplified fragment length polymorphism (AFLP) markers. Specifically, the study was conducted to determine the intra-specific variability of a total of 173 accessions, which were identified from five agro-ecological regions from Côte d'Ivoire (Centre, East, North and South). Then, the genetic diversity and relationships within accessions were studied using AFLP markers. This characterization using both morphological and AFLP markers was realized in order to ultimately build a reliable core collection. The discriminant analysis, using nine quantitative traits, reveals plant length and seeds number per fruit as discriminating characteristics. From the accessions used for the agromorphological study, 148 were able to be differentiated by the AFLP markers. A range of 52 to 113 bands were amplified per primer combination. As revealed by the analysis of molecular variance (AMOVA), 28% of the total variation resides among accessions and 72% occurs within populations. The AMOVA computed in order to differentiate cultivars, displayed the same trends when no prior grouping of accessions was considered. The differentiation within cultivar (97%) was more than that, among cultivars (3%). Tree topologies inferred by neighbour-joining analysis reflected no clear cut off grouping.
 
Date 2022-10-21
2023-01-06T17:03:56Z
2023-01-06T17:03:56Z
 
Type Journal Article
 
Identifier Gbotto, A.A.; Yao, N.K.; Kitavi, M.; Osama, S.K.; Habimana, R.; Koffi, K.K.; Zoro Bi, I.A. 2022. Genetic characterization of oleaginous bottle gourd (Lagenaria siceraria) germplasm from Côte d’Ivoire using agromorphological and molecular markers. Plant Genetic Resources: Characterization and Utilization. ISSN 1479-263X. 1–12.
https://hdl.handle.net/10568/126666
https://doi.org/10.1017/s1479262122000247
 
Language en
 
Rights Copyrighted; all rights reserved
Limited Access
 
Format 12 p.
 
Source Plant Genetic Resources