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<p style="text-align: justify;">Phytochemical analysis, biological activities, and GC profiling of extracts of some medicinal plant growing in Nepal</p>

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Title Statement <p style="text-align: justify;">Phytochemical analysis, biological activities, and GC profiling of extracts of some medicinal plant growing in Nepal</p>
 
Added Entry - Uncontrolled Name Banjara, Rama ; Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu 44613, Nepal
Sharma, Khaga Raj; Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu 44613, Nepal
Pokharel, Yuba Raj; Faculty of Life Science and Biotechnology, South Asian University, New Delhi 110021, India
 
Uncontrolled Index Term Phytochemistry
Antimicrobial; Antioxidant; Diabetes; DPPH; Jasminum humile; Medicinal plants
 
Summary, etc. <p style="text-align: justify;">The present study was aimed to determine the antioxidant, α amylase inhibition, and antibacterial activities on ten traditionally used medicinal plant extracts, namely <em>Cyperus rotundus</em>, <em>Citrus medica</em>, <em>Gaultheria fragrantissima</em>, <em>Jasminum humile</em>, <em>Osyris wightiana</em>, <em>Buddleja asiatica</em>, <em>Berberis aristata</em>, <em>Robus ellipticus</em>, <em>Schima wallichii</em>, and <em>Smilax ovalifolia </em>growing in Nepal. The bioactive fraction of <em>J. humile</em> was subjected for GC analysis. The free radical scavenging properties of plant extracts were assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and antibacterial activity was performed by the well diffusion method. The antidiabetic activity was assessed α amylase inhibition assay. The chemical compounds were isolated from the active plant fraction by silica column chromatography, and the collected fractions were analyzed by GC and FTIR. The phytochemical analysis showed that plant extracts were rich sources of secondary metabolites. The <em>in vitro </em>antioxidant activity showed IC<sub>50</sub> ranging from 30.57±0.02 to 155.65±0.10 µg/mL. The promising antioxidant activity was demonstrated by <em>S. wallichii </em>of IC<sub>50</sub> 30.57±0.02 µg/mL and <em>J. humile</em> 35.28±0.54 µg/mL, respectively whereas, the <em>S. ovalifolia</em>,<em> </em>exhibited the moderate antioxidant activity of IC<sub>50 </sub>155.65±0.10 µg/mL. The <em>J. humile </em>showed significant antidiabetic activity of IC<sub>50</sub> 59.4±23.47 µg/mL. The antidiabetic activities exhibited ranged from IC<sub>50</sub> of 77.29±2.05 (<em>S. wallichii </em>to 608.28±71.50 µg/mL (<em>C. rotundus</em>). The <em>R. ellipticus</em> showed maximum ZOI (22 mm) against the <em>Staphylococcus aureus </em>(ATCC 25923), whereas <em>J. humile</em> (20 mm), <em>O. wightiana</em> (18 mm), and <em>G. fragrantissima</em> (16 mm) showed moderate antibacterial activity against the <em>S. aureus</em>. The <em>C. rotundus</em>, <em>J. humile</em>, <em>S. ovalifolia</em>, <em>O. wightiana</em>, and <em>B. asiatica</em> showed promising antibacterial activity against <em>E. coli</em> (ATCC 25922) with ZOI 15, 17, 14, 17, and 18 mm respectively. These findings provide partial scientific support for traditional uses of these medicinal plants against diabetes and infectious diseases. Therefore, the <em>J. humile</em> could be a promising source of natural antidiabetic and antioxidant compounds that may be drug candidates for future drug development. To the best of our knowledge, this work is the first attempt to perform all these biological activities and phytochemical analyses growing in the particular region of Nepal.</p>
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2022-12-05 09:59:16
 
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http://op.niscair.res.in/index.php/IJNPR/article/view/39187
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 13, ##issue.no## 4 (2022): December 2022 (Ahead of Print)
 
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