Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)
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Title |
Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)
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Creator |
Roca, W.
Nolt, Barry L. Mafla, G. Roa E, Julio César Reyes, R. |
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Subject |
manihot esculenta
virus inhibition tissue culture apical meristems heat therapy elisa germplasm propagation cultivation disease control pest control plant tissues starch crops inhibicion de virus cultivo de tejidos meristemas apicales tratamiento termico germoplasma propagacion |
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Description |
Several techniques used to eliminate viruses from cassava are described, namely heat therapy, apical tissue culture, and micropropagation. Virological tests to index cassava viruses are also indicated as follows: ELISA, dsRNA, mechanical inoculation of leaf sap in plants on Chenopodium quinoa, and grafting trials. Clones submitted to pathogenic tests can be maintained in vitro, avoiding the recontamination with pathogenic organisms. The interrelations existing between the different forms of in vitro conservation of cassava germplasm and the elimination of diseases, micropropagation, distribution, and traditional conservation through seed and field plantations (basic vs. active collections) are indicated. The use of in vitro clonal propagation in cassava and the combination of different techniques to satisfy all multiplication needs are discussed, namely micropropagation through single-node cultures, multiple shoot culture, and somatic embryogenesis. Several examples are given that illustrate that rehabilitation of regional cassava cv. is a strategy to produce cassava in the poorer regions of the tropics in the short term. (CIAT)
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Date |
1991
2015-01-28T14:17:25Z 2015-01-28T14:17:25Z |
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Type |
Book Chapter
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Identifier |
ROCA, W.M.; NOLT, B.; MAFLA, G.; ROA, J.; REYES, R. 1991. Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz). In: Roca, William M.; Mroginski, Luis A. (eds.). Cultivo de tejidos en la agricultura: Fundamentos y aplicaciones. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. p. 403-420.
https://hdl.handle.net/10568/55095 https://books.google.com.co/books/p/pub-2347935248438357?id=EXijYNw55DUC&pg=PA401&source=gbs_selected_pages&cad=1#v=onepage&q&f=false |
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Language |
es
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Rights |
Open Access
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Format |
p. 403-420
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Publisher |
International Center for Tropical Agriculture
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