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Identification of F1 cassava (Manihot esculenta Crantz) progeny using microsatellite markers and capillary electrophoresis

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Title Identification of F1 cassava (Manihot esculenta Crantz) progeny using microsatellite markers and capillary electrophoresis
 
Creator Kyaligonza, V.
Kawuki, Robert S.
Ferguson, M.
Kaweesi, T.
Baguma, Yona K.
Vuzi, P.
 
Subject progeny
genetic markers
 
Description Generation of genetic diversity is necessary in improving on the potential of cassava when faced with various biotic and abiotic challenges. Presently, cassava breeders are breeding for a number of traits, such as drought tolerance, early root bulking, yield, starch, beta-carotene, protein, dry matter, pest and disease resistance, by relying on genetic diversity that exists in manihot esculenta germplasm. Controlled pollination is one of the main methods used to generate genetic diversity in cassava. However, the process of controlled pollination especially in an open field is prone to contamination by illegitimate pollen right from the time of pollination, seed collection, nursery bed establishment to planting of the trials. Therefore, authentication of the progeny obtained from cas-sava crosses is very important for genetic studies. Twelve informative microsatellite markers were used to verify the authenticity of 364 F1 progeny thought to come from four controlled parental crosses. The transmission of each allele at nine microsatellite loci was tracked from parents to progeny in each of the four Namikonga-derived F1 cassava families. Out of the 364 F1 progeny, 317 (87.1%) were true-to-type, 44 (12.1%) were a product of self-pollination and 3 (0.8%) were a product of open pollination. The consistency of the results obtained using microsatellite markers makes this technique a reliable tool for assessing the purity of progeny generated from cassava crosses.
 
Date 2014
2016-07-04T08:15:35Z
2016-07-04T08:15:35Z
 
Type Journal Article
 
Identifier Kyaligonza, V., Kawuki, R.S., Ferguson, M., Kaweesi, T., Baguma, Y. & Vuzi, P.(2014). Identification of F1 Cassava (Manihot esculenta Crantz) progeny using microsatellite markers and capillary electrophoresis. American Journal of Plant Sciences, 5, 119-125.
2158-2742
https://hdl.handle.net/10568/75939
https://doi.org/10.4236/ajps.2014.51015
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 119-125
application/pdf
 
Publisher Scientific Research Publishing, Inc.
 
Source American Journal of Plant Sciences