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QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population

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Title QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population
 
Creator Megerssa, Shitaye Homma
Ammar, Karim
Acevedo, Maricelis
Bergstrom, Gary Carlton
Dreisigacker, Susanne
Randhawa, Mandeep S.
Brown-Guedira, Gina
Ward, Brian
Sorrells, Mark Earl
 
Subject breeding
chromosomes
genetic markers
genotyping
greenhouses
maize
phenotypic variation
plant response
quantitative analysis
quantitative trait loci mapping
seedlings
single nucleotide polymorphism
stem rust
wheat
Basidiomycota
disease resistance
genetics
microbiology
plant breeding
 
Description Stem rust caused by the fungus Puccinia graminis f.sp. tritici Eriks. & E. Henn. (Pgt) threatens the global production of both durum wheat (Triticum turgidum L. ssp. durum (Desf.) Husnot) and common wheat (Triticum aestivum L.). The objective of this study was to evaluate a durum wheat recombinant inbred line (RIL) population from a cross between a susceptible parent ‘DAKIYE’ and a resistant parent ‘Reichenbachii’ developed by the International Center for the Improvement of Maize and Wheat (CIMMYT) 1) for seedling response to races JRCQC and TTRTF and 2) for field response to a bulk of the current Pgt races prevalent in Ethiopia and Kenya and 3) to map loci associated with seedling and field resistances in this population. A total of 224 RILs along with their parents were evaluated at the seedling stage in the Ethiopian Institute for Agricultural Research greenhouse at Debre Zeit, Ethiopia and in the EIAR and KALRO fields in Ethiopia and Kenya, for two seasons from 2019 to 2020. The lines were genotyped using the genotyping-by-sequencing approach. A total of 843 single nucleotide polymorphism markers for 175 lines were used for quantitative trait locus (QTL) analyses. Composite interval mapping (CIM) identified three QTL on chromosomes 3B, 4B and 7B contributed by the resistant parent. The QTL on chromosome 3B was identified at all growth stages and it explained 11.8%, 6.5%, 6.4% and 15.3% of the phenotypic variation for responses to races JRCQC, TTRTF and in the field trials ETMS19 and KNMS19, respectively. The power to identify additional QTL in this population was limited by the number of high-quality markers, since several markers with segregation distortion were eliminated. A cytological study is needed to understand the presence of chromosomal rearrangements. Future evaluations of additional durum lines and RIL families identification of durable adult plant resistance sources is crucial for breeding stem rust resistance in durum wheat in the future.
 
Date 2022-10-06
2023-01-09T10:10:38Z
2023-01-09T10:10:38Z
 
Type Journal Article
 
Identifier Megerssa, S. H., Ammar, K., Acevedo, M., Bergstrom, G. C., Dreisigacker, S., Randhawa, M., Brown-Guedira, G., Ward, B., & Sorrells, M. E. (2022). QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population. PLOS ONE, 17(10), e0273993. https://hdl.handle.net/10883/22296
1932-6203
https://hdl.handle.net/10568/126707
https://hdl.handle.net/10883/22296
https://doi.org/10.1371/journal.pone.0273993
https://figshare.com/articles/dataset/QTL_mapping_of_seedling_and_field_resistance_to_stem_rust_in_DAKIYE_Reichenbachii_durum_wheat_population/20326143
 
Language en
 
Rights CC0-1.0
Open Access
 
Format application/pdf
 
Publisher Public Library of Science
 
Source PLoS ONE