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Multi-drug resistant Staphylococcus aureus carriage in abattoir workers in Busia, Kenya

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Title Multi-drug resistant Staphylococcus aureus carriage in abattoir workers in Busia, Kenya
 
Creator Obanda, B.A.
Gibbons, C.L.
Fèvre, Eric M.
Bebora, L.
Gitao, G.
Ogara, W.
Wang, S.-H.
Gebreyes, W.
Ngetich, R.
Blane, B.
Coll, F.
Harrison, E.M.
Kariuki, S.
Peacock, S.J.
Cook, Elizabeth A.J.
 
Subject health
antimicrobial resistance
infectious diseases
biochemistry
microbiology
 
Description Abattoir workers have been identified as high-risk for livestock-associated Staphylococcus aureus carriage. This study investigated S. aureus carriage in abattoir workers in Western Kenya. Nasal swabs were collected once from participants between February-November 2012. S. aureus was isolated using bacterial culture and antibiotic susceptibility testing performed using the VITEK 2 instrument and disc diffusion methods. Isolates underwent whole genome sequencing and Multi Locus Sequence Types were derived from these data. S. aureus (n = 126) was isolated from 118/737 (16.0%) participants. Carriage was higher in HIV-positive (24/89, 27.0%) than HIV–negative participants (94/648, 14.5%; p = 0.003). There were 23 sequence types (STs) identified, and half of the isolates were ST152 (34.1%) or ST8 (15.1%). Many isolates carried the Panton-Valentine leucocidin toxin gene (42.9%). Only three isolates were methicillin resistant S. aureus (MRSA) (3/126, 2.4%) and the prevalence of MRSA carriage was 0.4% (3/737). All MRSA were ST88. Isolates from HIV-positive participants (37.0%) were more frequently resistant to sulfamethoxazole/trimethoprim compared to isolates from HIV-negative participants (6.1%; p < 0.001). Similarly, trimethoprim resistance genes were more frequently detected in isolates from HIV-positive (81.5%) compared to HIV-negative participants (60.6%; p = 0.044). S. aureus in abattoir workers were representative of major sequence types in Africa, with a high proportion being toxigenic isolates. HIV-positive individuals were more frequently colonized by antimicrobial resistant S. aureus which may be explained by prophylactic antimicrobial use.
 
Date 2022-12-01
2022-12-02T09:00:16Z
2022-12-02T09:00:16Z
 
Type Journal Article
 
Identifier Obanda, B.A., Gibbons, C.L., Fèvre, E.M., Bebora, L., Gitao, G., Ogara, W., Wang, S.-H., Gebreyes, W., Ngetich, R., Blane, B., Coll, F., Harrison, E.M., Kariuki, S., Peacock, S.J. and Cook, E.A.J. 2022. Multi-drug resistant Staphylococcus aureus carriage in abattoir workers in Busia, Kenya. Antibiotics 11(12): 1726.
2079-6382
https://hdl.handle.net/10568/125757
https://doi.org/10.3390/antibiotics11121726
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 1726
 
Publisher MDPI
 
Source Antibiotics