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Validation of KASP markers associated with cassava mosaic disease resistance, storage root dry matter and provitamin A carotenoid contents in Ugandan cassava germplasm

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Title Validation of KASP markers associated with cassava mosaic disease resistance, storage root dry matter and provitamin A carotenoid contents in Ugandan cassava germplasm
 
Creator Esuma, W.
Eyoo, O.
Gwandu, F.
Mukasa, S.
Alicai, T.
Ozimati, A.
Nuwamanya, E.
Rabbi, I.Y.
Kawuki, R.
 
Subject genetic gain
cassava
manihot esculenta
genetic markers
genotypes
uganda
 
Description Open Access Journal; Published online: 23 Nov 2022
Introduction
The intrinsic high heterozygosity of cassava makes conventional breeding ineffective for rapid genetic improvement. However, recent advances in next-generation sequencing technologies have enabled the use of high-density markers for genome-wide association studies, aimed at identifying single nucleotide polymorphisms (SNPs) linked to major traits such as cassava mosaic disease (CMD) resistance, dry matter content (DMC) and total carotenoids content (TCC). A number of these trait-linked SNPs have been converted to Kompetitive allele-specific polymerase chain reaction (KASP) markers for downstream application of marker assisted selection.
Methods
We assayed 13 KASP markers to evaluate their effectiveness in selecting for CMD, DMC and TCC in 1,677 diverse cassava genotypes representing two independent breeding populations in Uganda.
Results
Five KASP markers had significant co-segregation with phenotypes; CMD resistance (2), DMC (1) and TCC (2), with each marker accounting for at least 30% of the phenotypic variation. Markers located within the chromosomal regions for which strong marker-trait association loci have been characterised (chromosome 12 markers for CMD, chromosome 1 markers for DMC and TCC) had consistently superior ability to discriminate the respective phenotypes.
Discussion
The results indicate varying discriminatory abilities of the KASP markers assayed and the need for their context-based use for MAS, with PSY2_572 particularly effective in selecting for high TCC. Availing the effective KASP markers on cost-effective genotyping platforms could facilitate practical implementation of marker-assisted cassava breeding for accelerated genetic gains for CMD, DMC and provitamin A carotenoids.
 
Date 2022-11-23
2023-01-18T12:01:37Z
2023-01-18T12:01:37Z
 
Type Journal Article
 
Identifier Esuma, W., Eyoo, O., Gwandu, F., Mukasa, S., Alicai, T., Ozimati, A., ... & Kawuki, R. (2022). Validation of KASP markers associated with cassava mosaic disease resistance, storage root dry matter and provitamin A carotenoid contents in Ugandan cassava germplasm. Frontiers in Plant Science, 13: 1017275, 1-12.
1664-462X
https://hdl.handle.net/10568/127385
https://doi.org/10.3389/fpls.2022.1017275
BIOTECH & PLANT BREEDING
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 1-12
application/pdf
 
Source Frontiers in Plant Science