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Mitochondrial genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava in Lao PDR

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Title Mitochondrial genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava in Lao PDR
 
Creator Leiva, Anne
Chittarath, Khonesavanh
López Alvarez, Diana
Vongphachanh, Pinkham
Gómez Jiménez, Maria Isabel
Sengsay, Somkhit
Wang, Xiao-Wei
Rodríguez, Rafael
Newby, Jonathan Craig
Cuéllar, Wilmer Jose
 
Subject bemisia tabaci
plant viruses
disease control
mitochondrial genetics
virus de las plantas
control de enfermedades
genética mitocondrial
 
Description Cassava Mosaic Disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV), has rapidly spread in Southeast Asia (SEA) since 2016. Recently it has been documented in Lao PDR. Previous reports have identified whitefly species of B. tabaci as potential vectors of CMD in SEA, but their occurrence and distribution in cassava fields is not well known. We conducted a countrywide survey in Lao PDR for adult whiteflies in cassava fields, and determined the abundance and genetic diversity of the B. tabaci species complex using mitochondrial cytochrome oxidase I (mtCOI) sequencing. In order to expedite the process, PCR amplifications were performed directly on whitefly adults without DNA extraction, and mtCOI sequences obtained using nanopore portable-sequencing technology. Low whitefly abundances and two cryptic species of the B. tabaci complex, Asia II 1 and Asia II 6, were identified. This is the first work on abundance and genetic identification of whiteflies associated with cassava in Lao PDR. This study indicates currently only a secondary role for Asia II in spreading CMD or as a pest. Routine monitoring and transmission studies on Asia II 6 should be carried out to establish its potential role as a vector of SLCMV in this region.
 
Date 2022-09-22
2022-09-23T09:40:42Z
2022-09-23T09:40:42Z
 
Type Journal Article
 
Identifier Leiva, A.; Chittarath, K.; Lopez-Alvarez, D.; Vongphachanh, P.; Gomez, M.; Sengsay, S.; Wang, X-W.; Rodriguez, R.; Newby, J.; Cuellar, W.J. (2022) Mitochondrial genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava in Lao PDR. Insects 13: 861. 13 p. ISSN: 2075-4450
2075-4450
https://hdl.handle.net/10568/121932
https://doi.org/10.3390/insects13100861
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 13 p.
application/pdf
 
Publisher MDPI AG
 
Source Insects